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Figure 2. Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. (A) GLUT1, GLUT2, GLUT3, and <t>GLUT4</t> protein levels after 24 h of the in vitro addition of different concentrations
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Figure 2. Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. (A) GLUT1, GLUT2, GLUT3, and <t>GLUT4</t> protein levels after 24 h of the in vitro addition of different concentrations
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Figure 2. Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. (A) GLUT1, GLUT2, GLUT3, and GLUT4 protein levels after 24 h of the in vitro addition of different concentrations

Journal: International journal of molecular sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells.

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Figure 2. Effects of AdipoRon on the transport of glucose in rat ovarian granulosa cells. (A) GLUT1, GLUT2, GLUT3, and GLUT4 protein levels after 24 h of the in vitro addition of different concentrations

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal antirabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: In Vitro

Figure 4. Transcriptome data and correlation analysis of the expression of glucose transporters and the adipokine system in rat ovaries. (A) Volcanic map analysis of transcriptome data. Blue: represents no significant difference. (B) KEGG pathway analysis; (C) correlation analysis of genes related to the glucose metabolism signaling pathway and adipokine signaling pathway. (D) Gene transcription levels of Slc2a1, Slc2a2, Slc2a3, Slc2a4, Adipor1, and Adipor2 in the ovaries of rats in the eCG-treated and blank control groups. (E) Protein levels of GLUT1, GLUT2, GLUT3, GLUT4, AdipoR1, and AdipoR2 in the ovaries of rats in the eCG-treated and blank control groups. The error bars represent means ± SEM (n = 3, each group). * Statistically significant values (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001); ns represents no significance.

Journal: International journal of molecular sciences

Article Title: Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells.

doi: 10.3390/ijms25105155

Figure Lengend Snippet: Figure 4. Transcriptome data and correlation analysis of the expression of glucose transporters and the adipokine system in rat ovaries. (A) Volcanic map analysis of transcriptome data. Blue: represents no significant difference. (B) KEGG pathway analysis; (C) correlation analysis of genes related to the glucose metabolism signaling pathway and adipokine signaling pathway. (D) Gene transcription levels of Slc2a1, Slc2a2, Slc2a3, Slc2a4, Adipor1, and Adipor2 in the ovaries of rats in the eCG-treated and blank control groups. (E) Protein levels of GLUT1, GLUT2, GLUT3, GLUT4, AdipoR1, and AdipoR2 in the ovaries of rats in the eCG-treated and blank control groups. The error bars represent means ± SEM (n = 3, each group). * Statistically significant values (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001); ns represents no significance.

Article Snippet: The goat polyclonal anti-rabbit AdipoR2 (sc-46751, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse AdipoR1 (sc46748, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-rabbit GLUT1 (21829-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT2 (20436-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal antirabbit GLUT3 (20403-1-AP, Proteintech Biotechnology, Wuhan, China), rabbit polyclonal anti-mouse GLUT4 (sc-53566, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-mouse vinculin (sc-73614, Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit polyclonal anti-mouse adiponectin (ab181281, abcam Biotechnology, Cambridge, UK) were also used.

Techniques: Expressing, Control